itga5 protein  (MedChemExpress)

Journal: Phytomedicine : international journal of phytotherapy and phytopharmacology

Article Title: 13-Methylpalmatine alleviates bleomycin-induced pulmonary fibrosis by suppressing the ITGA5/TGF-β/Smad signaling pathway.

doi: 10.1016/j.phymed.2025.156545

Figure Lengend Snippet: Fig. 5. Transcriptomics analysis, molecular docking, molecular dynamics simulation, and SPR analysis. A Volcano map of the 147 intersecting genes. B PPI network. C Kegg pathway. D Schematic diagram of molecular docking of 13-Me-PLT with ITGA5. E RMSD of ITGA5 with 13-Me-PLT. F Spacing of ITGA5 and 13-Me-PLT binding sites (Dock site-ligand). G Interaction of ITGA5 with 13-Me-PLT. H SPR analysis of ITGA5 and 13- Me-PLT.

Article Snippet: The CM7 chip was selected and ITGA5 protein (50μg, shanghai, MCE) was diluted to 50 μg/ml using sodium acetate with a pH value of 4.0 and the protein was coupled to the chip surface (10,988 RU).

Techniques: Binding Assay

Journal: Phytomedicine : international journal of phytotherapy and phytopharmacology

Article Title: 13-Methylpalmatine alleviates bleomycin-induced pulmonary fibrosis by suppressing the ITGA5/TGF-β/Smad signaling pathway.

doi: 10.1016/j.phymed.2025.156545

Figure Lengend Snippet: Fig. 6. Effects of 13-Me-PLT on BLM-induced ITGA5 and TGF-β/Smad signaling pathways in mice and TGF-β/Smad signaling pathways in Ang II-stimulated MRC5 cells. A, B Immunohistochemical staining of ITGA5 in lung tissue and relative staining intensity analysis. Scale bar = 50 μm; n = 3. C, D mRNA levels of ITGA5 and TGF-β1 in lung tissue (n = 5–6). E-H Representative blotting and quantitative analysis of protein levels of ITGA5, TGF-β1 and p-Smad3 in lung tissue (n = 6). I, J Immu nofluorescence staining and relative fluorescence intensity analysis of ITGA5 in lung tissue. Scale bar = 100 μm; n = 3. K, l mRNA levels of ITGA5 and TGF-β1 in cells of each group (n = 4–5). M-O Quantitative analysis of protein levels of ITGA5, TGF-β1 and p-Smad3 in cells of each group (n = 4). P, Q Relative fluorescence intensity analysis of ITGA5 in cells of each group (n = 3). Data are expressed as SEM ± mean. **p < 0.01, ***p < 0.001 vs. Control, #p < 0.05, ##p < 0.01, ###p < 0.001 vs. BLM or AngII.

Article Snippet: The CM7 chip was selected and ITGA5 protein (50μg, shanghai, MCE) was diluted to 50 μg/ml using sodium acetate with a pH value of 4.0 and the protein was coupled to the chip surface (10,988 RU).

Techniques: Protein-Protein interactions, Immunohistochemical staining, Staining, Fluorescence, Control

Journal: Phytomedicine : international journal of phytotherapy and phytopharmacology

Article Title: 13-Methylpalmatine alleviates bleomycin-induced pulmonary fibrosis by suppressing the ITGA5/TGF-β/Smad signaling pathway.

doi: 10.1016/j.phymed.2025.156545

Figure Lengend Snippet: Fig. 7. Effects of silencing ITGA5 on fibrosis-related factors and the TGF-β/Smad signaling pathway in each group of cells. A-E mRNA levels of FN1, Collagen I, α-SMA, ITGA5, and TGF-β1 in cells of each group (n = 4). F-H Representative blotting and quantitative analysis of FN1 and Collagen I protein levels in cells of each group (n = 4). I-l Representative blotting and quantitative analysis of protein levels of ITGA5, TGF-β1 and p-Smad3 in each group of cells (n = 4). M, N Immunofluorescence staining and quantitative analysis of mean fluorescence intensity of α-SMA in each group of cells (n = 3). Scale bar = 100 μm. O, P Immunofluorescence staining and quantitative analysis of mean fluorescence intensity of ITGA5 in each group of cells (n = 3), scale bar = 100 μm. Q, R Transwell detection of cell migration numbers in each group of cells and statistical analysis of migration data. Scale bar = 75 μm; n = 3. Data are expressed as SEM ± mean. **p < 0.01,***p < 0.001 vs. Control, #p < 0.05, ##p < 0.01, ###p < 0.001 vs. AngII.

Article Snippet: The CM7 chip was selected and ITGA5 protein (50μg, shanghai, MCE) was diluted to 50 μg/ml using sodium acetate with a pH value of 4.0 and the protein was coupled to the chip surface (10,988 RU).

Techniques: Immunofluorescence, Staining, Fluorescence, Migration, Control

Journal: Phytomedicine : international journal of phytotherapy and phytopharmacology

Article Title: 13-Methylpalmatine alleviates bleomycin-induced pulmonary fibrosis by suppressing the ITGA5/TGF-β/Smad signaling pathway.

doi: 10.1016/j.phymed.2025.156545

Figure Lengend Snippet: Fig. 8. Effect of 13-Me-PLT on fibrosis-related indices and TGF-β/Smad signaling pathway in various groups of cells after overexpression of ITGA5. A-E mRNA levels of FN1, Collagen I, α-SMA, ITGA5, and TGF-β1 in cells of each group (n = 4). F-H Representative blotting and quantitative analysis of FN1 and Collagen I protein levels in cells of each group (n = 4). I-l Representative blotting and quantitative analysis of protein levels of ITGA5, TGF-β1 and p-smad3 in each group of cells (n = 4). M, N Immunofluorescence staining and quantitative analysis of mean fluorescence intensity of α-SMA in each group of cells (n = 3). Scale bar = 100 μm. O, P Immunofluorescence staining and quantitative analysis of mean fluorescence intensity of α-SMA in each group of cells (n = 3). Scale bar = 100 μm. Q, R Transwell detection of cell migration numbers in each group of cells and statistical analysis of migration data (n = 3). Scale bar = 75 μm. Data are expressed as SEM ± mean. **p < 0.01,***p < 0.001 vs. Control, #p < 0.05, ##p < 0.01, ###p < 0.001 vs. P-ITGA5.

Article Snippet: The CM7 chip was selected and ITGA5 protein (50μg, shanghai, MCE) was diluted to 50 μg/ml using sodium acetate with a pH value of 4.0 and the protein was coupled to the chip surface (10,988 RU).

Techniques: Over Expression, Immunofluorescence, Staining, Fluorescence, Migration, Control

Journal: Phytomedicine : international journal of phytotherapy and phytopharmacology

Article Title: 13-Methylpalmatine alleviates bleomycin-induced pulmonary fibrosis by suppressing the ITGA5/TGF-β/Smad signaling pathway.

doi: 10.1016/j.phymed.2025.156545

Figure Lengend Snippet: Fig. 9. Schematic representation of possible targets and molecular mechanisms of 13-Me-PLT for the treatment of IPF. 13-Me-PLT ameliorates pulmonary fibrosis by inhibiting ITGA5, suppressing activation of the TGF-β/Smad signaling pathway, and decreasing phosphorylation of samd3, which reduces the deposition of ECM (collagens, fibronectin, α-SMA, etc.). 13-Me-PLT: 13-methylpalmatine; ITGA5: integrin alpha-5; ECM : extracellular matrix; TGF-β1: transforming growth factor-β1; TβR-I: Transforming Growth Factor Beta-Receptor Type I; TβR-II: Transforming Growth Factor Beta-Receptor Type II.

Article Snippet: The CM7 chip was selected and ITGA5 protein (50μg, shanghai, MCE) was diluted to 50 μg/ml using sodium acetate with a pH value of 4.0 and the protein was coupled to the chip surface (10,988 RU).

Techniques: Activation Assay, Phospho-proteomics

Journal: PLoS ONE

Article Title: An innovative strategy to identify new targets for delivering antibodies to the brain has led to the exploration of the integrin family

doi: 10.1371/journal.pone.0274667

Figure Lengend Snippet: Anti-integrin antibodies apparent affinities to different integrin subunits investigated by ELISA.

Article Snippet: Recombinant integrin proteins were purchased from OriGene for human monomer α3, α5 and β1 (tp320975, tp301151 and tp303818), from GeneTex for human monomer α4 (GTX48181), from R&D Systems for human and mouse dimer α3β1, α4β1 and α5β1 (2840-a3, 3230-a5, 5668-a4, 7728-a5, 9374-a3) for human ALCAM 656-AL, from Sino Biological for human α5β1 (CT-014-H2508H), from Abcam for Striatin3 (ab162295) Antibodies were purchased from antibodies-online (natalizumab ABIN5668196), from Abcam (Anti-VE Cadherin ab33168), from BD Biosciences (553715), from BioLegend (343802 and MFR5 103801), from Interchim (DCABH-8217), from Invitrogen (14-0299-82, MA5-17103, MA1-25298), from Millipore (MABT409, MABT199, MAB2079Z) from Novus Biological (NBP2-52708), from Proteintech (66070-1-Ig), from R&DSystems (MAB1345), from Sigma-Aldrich (MAB1965), from ThermoFisher Scientific (Anti-ZO-1 #61–7300; Anti-Occludin #33–1500; all other antibodies were produced in-house by Sanofi Biological Research. hCMEC/D3 cells were obtained from Cedarlane.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Nature Communications

Article Title: Crystal structure and cellular functions of uPAR dimer

doi: 10.1038/s41467-022-29344-y

Figure Lengend Snippet: a Cell proliferation curve was generated by analyzing images of DPC (digital phase contrast) for different cell lines as indicated using Operetta CLS software. The DPC channel was recorded every half an hour for 60 h on HCS in the cell culture condition (37 °C, 5%CO 2 ). Data are presented as mean ± SD. Source data are provided as a Source Data file. b Microscopic images of the cytoskeleton (green) of uPAR mutants fused with mRuby3 (red) stable cell lines. The cells were treated with Hoechst (blue) for 10 min before fixation and incubated with Actin-Tracker (green) for 1 h after 10-min permeabilization with 1% Triton X-100. Scale bars, 50 µm. c Immuno-analysis of mRuby3-tagged uPAR mutants stably expressed in 293T cells. The mRuby3 293T cells were treated with 10 mM MnCl 2 for 30 min as a positive control. Cell lysates were separated and analyzed by immunoblotting using polyclonal anti-uPAR antibody (α-uPAR), anti-ERK1/2 (α-ERK1/2), anti-phospho-ERK1/2 (α-p-ERK1/2), and anti-GAPDH (α-GAPDH). Source data are provided as a Source Data file. d Microscopic images of the cytoskeleton (green) of uPAR mutants fused with mRuby3 (red) stable cell lines treated with integrin for 3 h, then stained as b . Scale bars, 50 µm.

Article Snippet: Integrin α5β1 protein (CT014-H2508H) was purchased from SinoBiological.

Techniques: Generated, Software, Cell Culture, Stable Transfection, Incubation, Positive Control, Western Blot, Staining